Article metadata
Abstract
Element or context:
<abstract>
Requirements:
The abstract of an article is the abstract submitted by the author and therefore will be the same language (English or French) as the rest of the article. The following tagging requirements are for the abstract. For the translated abstract, please see Abstract, translated.
- Place the abstract inside <abstract> within <p> tags
Example:
<abstract>
<p>The chestnut blight fungus, <italic>Cryphonectria parasitica,</italic> and its hypovirus present a useful model system for investigating the mechanisms of hypoviral infection. To identify gene products associated with fungal pathogenicity and hypoviral regulation, a proteomic analysis of the virus-free EP155/2 strain and its isogenic virus-infected UEP1 strain in response to tannic acid (TA), which is abundant in the bark of chestnut trees, was attempted. In this study, pre-treatment of mycelia grown on TA-supplemented media was developed for proteomic analysis. Approximately 704 proteins from the mycelia of the EP155/2 strain were reproducibly present in three independent extractions. Among these, 111 and 79 spots were found to be responsive to hypovirus infection and TA supplementation, respectively. The TA-grown UEP1 strain yielded 28 spots showing different expression pattern from that of untreated UEP1. Thirty protein spots showing considerable differences in spot density were selected for further analysis. Hybrid tandem LC-MS/MS spectrometry of the 30 selected protein spots revealed that 29 were identified, while 1 were unidentified. Among the identified 29 proteins, fifteen were metabolic enzymes and five were stress-related including four heat-shock proteins and one glutathione-S-transferase. Five were signaling and cellular process-related proteins, two were structural proteins, and two matched to hypothetical genes.</p> </abstract>